Identification: Mould identification

Discussion in 'Fungi, Lichens and Slime Molds' started by fungi99, Mar 24, 2007.

  1. fungi99

    fungi99 Active Member

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    Hi people,

    I'm new here but I'm yet appreciating your useful work in the botanics field.
    So, first of all, a big thank you!

    I picked up a small soil sample and diluted it in water in 1/100 proportion;
    a sterile loop of wire was wetted in this solution and streaked across
    a Sabouraud's agar plate (for those who aren't familiar with this, it's an
    agar with low pH so that it inhibits the growth of most bacteria, plus
    the antibiotic gentamicin).
    Being familiar with the bunch of fungi which would grow out of these
    kind of samples, I decided to incubate the plate at relatively low temperatures
    (9°C,49°F) so that only a limited amount of species would grow.
    After some time, something of wooly aspect came out.

    Here I attach a picture of this colonia after about 3 days of incubation:
    about 30 mm x 30 mm, 5 mm in thickness

    Thank you for help
     

    Attached Files:

  2. Geastrum

    Geastrum Active Member

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    It looks like a species of the Zygomycete Rhizopus (possibly Rhizopus stolonifer?)
     
  3. fungi99

    fungi99 Active Member

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    I don't know...some sources says it needs warm places to grow...thank you anyway!
    If it's really Rhizopus stolonifer, seems like something harmful, as it produces allergic reactions on both animal and human mucus passages.

    Would it reproduced by hyphae fragmentation? Like I pick these wire-like mould structures and seed it on some other media?

    Thank you in advance
     
  4. Illecippo

    Illecippo Active Member

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    I don't know exactely (not an expert in this matter), but ypur experiment is very nice.

    Claps for you!!!

    Nico
     
  5. fungi99

    fungi99 Active Member

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    thank you !

    if someone can answer my question or deepen the subject, I'd really appreciate this.
     
  6. Daniel Mosquin

    Daniel Mosquin Paragon of Plants UBC Botanical Garden Forums Administrator Forums Moderator 10 Years

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    It's been a long, long time since I've done any of this sort of lab work (13-14 years, I reckon). I'd like to help, but I don't have the resources available to me (books, lab manuals) to jog my memory about what will be required to figure it out.

    Have you considered contacting fungi lab instructors at your local universities or colleges?
     
  7. fungi99

    fungi99 Active Member

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    I can't contact them at the moment because they're travelling from one seminar to another in europe.
    thank you for your interest anyway. If I'll grow some other fungi on agar, I'll keep you informed.

    regards
     

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