Hi people, I'm new here but I'm yet appreciating your useful work in the botanics field. So, first of all, a big thank you! I picked up a small soil sample and diluted it in water in 1/100 proportion; a sterile loop of wire was wetted in this solution and streaked across a Sabouraud's agar plate (for those who aren't familiar with this, it's an agar with low pH so that it inhibits the growth of most bacteria, plus the antibiotic gentamicin). Being familiar with the bunch of fungi which would grow out of these kind of samples, I decided to incubate the plate at relatively low temperatures (9°C,49°F) so that only a limited amount of species would grow. After some time, something of wooly aspect came out. Here I attach a picture of this colonia after about 3 days of incubation: about 30 mm x 30 mm, 5 mm in thickness Thank you for help
I don't know...some sources says it needs warm places to grow...thank you anyway! If it's really Rhizopus stolonifer, seems like something harmful, as it produces allergic reactions on both animal and human mucus passages. Would it reproduced by hyphae fragmentation? Like I pick these wire-like mould structures and seed it on some other media? Thank you in advance
I don't know exactely (not an expert in this matter), but ypur experiment is very nice. Claps for you!!! Nico
It's been a long, long time since I've done any of this sort of lab work (13-14 years, I reckon). I'd like to help, but I don't have the resources available to me (books, lab manuals) to jog my memory about what will be required to figure it out. Have you considered contacting fungi lab instructors at your local universities or colleges?
I can't contact them at the moment because they're travelling from one seminar to another in europe. thank you for your interest anyway. If I'll grow some other fungi on agar, I'll keep you informed. regards