Dr, How do you do? I am a post graduate student. I try to have a Hypocotyl derived callus of Medicago sativa. I use Ms media, NAA, and Cytokinin. Could you help me and answer these questions: what are the best concentrations of these hormone to have callus? How can i suggest (predict) the suitable hormone, concentrations and media in related to the type of plant species [*]Monocot or Dicot? [*]Angiosperm or Gymnosperm? [*]Explant Thank you Very Much Diana Raie
